Many specific techniques are used for the isolation of the DNA sample. Some of the extra chromosomal DNA is easier to isolate. These are done by the process of cell lysis and are adhered by the precipitation of the DNA. These are used for the process to trap chromosomal DNA. The next stage is the detection of the DNA. Use of diphenylamine (DPA) is used to confirm the presence of DNA. These involve the process of chemical hydrolysis. The DNA concentration is determined by the measure of the intensity of the absorbance of the solution. This is done by the use of the spectrophotometer. They are then compared to the standard curve of DNA concentrations (Taberlet, Waits and Luikart, 1999). These are measured based on the intensity of the absorbance of the DNA solutions. In these cases, the DNA absorbs the ultraviolet light between 260 to 280 nm. The aromatic proteins are about 280 nm. The purity of the sample of the DNA has the ratio of 1.8. The DNA is quantified by the process of cutting DNA with restriction enzymes. These are then made to run under the agarose gel. They are stained with ethidium bromide or different stain. These are compared with the intensity of DNA. The DNA markers are markers of the known concentration. Southern blot technique is then used. This process is done for the quantified DNA that is isolated and examined using PCR or RFLP analysis. These are then developed as procedures to allow the differentiation of repeated sequences in the genome. Ultimately, the techniques that are used for the process of DNA extraction and analysis depend on the objective of the experiment and the purpose of experimentation.