论文代写:DNA检测技术

大学论文代写

论文代写:DNA检测技术

许多特定的技术被用于分离DNA样本。一些额外的染色体DNA更容易分离。这些都是由细胞溶解过程完成的,并且是由DNA的沉淀所粘附的。这些被用于诱捕染色体DNA的过程。下一阶段是DNA检测。使用二苯胺(DPA)来证实DNA的存在。这涉及到化学水解的过程。DNA浓度是由溶液吸收度的测量值决定的。这是通过使用分光光度计来完成的。然后将它们与DNA浓度的标准曲线进行比较(Taberlet,等待和Luikart,1999)。这些都是根据DNA溶液的吸收强度来测量的。在这些情况下,DNA会吸收260到280纳米之间的紫外线。芳香蛋白大约有280纳米。DNA样本的纯度为1.8。DNA通过限制酶切DNA的过程被量化。然后这些被制成在琼脂糖凝胶下运行。它们被溴化乙锭或不同的染色剂染色。这些与DNA的强度相比。DNA标记物是已知浓度的标记物。然后使用Southern blot技术。这一过程是用PCR或RFLP分析分离和检查的量化DNA进行的。然后,这些被开发成程序,允许在基因组中区分重复序列。最终,用于DNA提取和分析过程的技术依赖于实验的目的和实验目的。

论文代写:DNA检测技术

Many specific techniques are used for the isolation of the DNA sample. Some of the extra chromosomal DNA is easier to isolate. These are done by the process of cell lysis and are adhered by the precipitation of the DNA. These are used for the process to trap chromosomal DNA. The next stage is the detection of the DNA. Use of diphenylamine (DPA) is used to confirm the presence of DNA. These involve the process of chemical hydrolysis. The DNA concentration is determined by the measure of the intensity of the absorbance of the solution. This is done by the use of the spectrophotometer. They are then compared to the standard curve of DNA concentrations (Taberlet, Waits and Luikart, 1999). These are measured based on the intensity of the absorbance of the DNA solutions. In these cases, the DNA absorbs the ultraviolet light between 260 to 280 nm. The aromatic proteins are about 280 nm. The purity of the sample of the DNA has the ratio of 1.8. The DNA is quantified by the process of cutting DNA with restriction enzymes. These are then made to run under the agarose gel. They are stained with ethidium bromide or different stain. These are compared with the intensity of DNA. The DNA markers are markers of the known concentration. Southern blot technique is then used. This process is done for the quantified DNA that is isolated and examined using PCR or RFLP analysis. These are then developed as procedures to allow the differentiation of repeated sequences in the genome. Ultimately, the techniques that are used for the process of DNA extraction and analysis depend on the objective of the experiment and the purpose of experimentation.