論文代寫-LRRC8a對血壓的影響研究

英国论文代写
论文通代写作者简介

英国论文代写 论文通

英国论文通 ASSIGNMENT PASS - 论文代写以独特的英国论文代写.英国essay代写和assignment代写专业服务理念,尝试创新的代写形式赢得了英国留学生的口碑.我们代写团队对于代写论文采取多样化的手段.做到了代写论文的原创性和对论文抄袭的杜绝.

19/01/2020

論文代寫-LRRC8a對血壓的影響研究。本研究的第二個目的是通過分析內皮細胞來了解LRRC8a對血壓的潛在影響。本實驗中應測量WT小鼠的血壓,並測量LRRC8a KO小鼠的內皮細胞。為此,將測量基線,血管緊張素ii輸注通過滲透微型泵。滲透微型泵將用於誘發高血壓。通過尾袖血壓測量和植入的主動脈血壓端粒來測量血壓。在此分析之後,我們將對移出的主動脈進行血管反應性研究,分別對p-eNOS、eNOS、pAKT1、pAKT2、AKT1、AKT2、p-p66shc進行免疫染色。然後用內皮一氧化氮和氧化劑對其進行定量。將采用熒光和化學發光方法。接下來論文范文論文代寫-LRRC8a對血壓的影響研究分享給留學生閱讀。

The primary objective of this current proposal is to determine the mechanism by which the LRRC8a regulates endothelium dependent vasorelaxation and blood pressure. In order to test the above hypothesis of LRRC8a, there will be two specific aims that will be analyzed for the research. It is the investigation of the LRRC8a-p66shc-PI3K-AKT-eNOS signaling in endothelium. For this, there should be analysis of the LRRC8a for activation of PI3K-AKT-eNOS and for the suppression of the p66shc pathway. This is done by measuring i pAKT1, pAKT2, AKT1, AKT1, p-eNOS, eNOS, and p-p66shc +/- LRRC8a +/- p66shc under basal resting conditions and in response to endothelial cell stretching on deformable membranes using the FlexCell System. Subsequently, there will be experimentation on HUVEC and in arterial endothelial cells +/- Ad-shSCR/LRRC8a +/- RNAi for p66shc. Added to this the primary cultures of aortic endothelial cells will be isolated from WT and endothelial LRRC8a KO of mice and transgenic mice over expressing shRNA to p66shc. From this, there will be determination made about the LRRC8a forms a molecular complex with p66shc. This is done by co-immunoprecipitation and co-localization studies in endothelial cells.

The second aim of this research is to understand the underlying implication of the LRRC8a on blood pressure by analyzing the endothelial cell. In this sceaniro, there should be measurement of the blood pressure in WT and the LRRC8a KO mice endothelial cells should be measures. For this, there will be measurement of the baseline, Angiotensin-II infusion via osmotic mini-pumps. Osmotic mini-pumps will be used to induce conditions of hypertension. Blood pressure will be measured by using Tail-cuff BP measurement and implantable aortic BP telomeres. If it is observed that LRRC8a KO mice develop HTN then they will be cross analyzed with p66shcRNAi mice. This p66shcRNAi mouse is found to have a global knockdown of p66shc. This is done to determine if this phenotype can be found in LRRC8a KO/p66shcRNAi mice. Subsequent to this analysis the vascular reactivity studies on explanted aortas, immunostaining of p-eNOS, eNOS, pAKT1, pAKT2, AKT1, AKT2, p-p66shc will be done. This would then be and quantified with endothelial NO and oxidants. Fluorescence and chemiluminescence methodologies will be performed. Previous literature of endothelial VRAC has been analyzed over the past 16 years. There is a considerable lack of knowledge about the ion channels. Using the adenoviral shRNA-mediated LRRC8a knock-down it can be established that LRRC8a is required for the volume-regulated anion current (VRAC) in human umbilical vein endothelial cells

論文范文論文代寫-LRRC8a對血壓的影響研究就為留學生分享到這裏。留學生論文不會寫怎么辦?論文代寫推薦英國論文通AssignmentPass網站平台,此論文代寫平台是英國信譽高的留學教育機構,論文寫作專業信譽高,而且論文代寫價格公平合理,為留學生們提供畢業論文代寫、essay代寫、assignment代寫等論文服務,英國論文通AssignmentPass豐富的論文代寫經驗值得留學生選擇和信賴!

相关的论文代写话题 . . .

如何选择英国论文代写推荐的公司?

如何选择英国论文代写推荐的公司?

我们在网络上搜索“英国论文代写推荐”这个关键词,会出现很多论文代写公司,这时候我们就要做好对比工作,选择一个规模比较大的公司合作,同时也做好性价比的比较工作,这样我们的论文才会更加有保障。那么,论文代写机构这么多,如何选择英国论文代写推荐的公司?下面听听英国代写老师的讲解。

英国论文代写价格为什么差距大?

英国论文代写价格为什么差距大?

英国论文代写价格是我们十分注意了解的,每一个人都想找一个性价比高的代写机构为我们服务。我们都知道英国论文要求比较高,留学生写起来很吃力,一般找代写都比较愿意找英国本土写手,可是网络上不同代写机构收费差距很大。那么,英国论文代写价格为什么差距大?下面我们来听听英国代写老师是如何说的。

英国essay代写哪家靠谱呢?

英国essay代写哪家靠谱呢?

我们在找英国essay代写机构的时候,往往是通过网络来寻找,这样双方不用见面,直接说出我们的要求,对方就可以帮我们写作了,并且不会透漏我们的个人信息。而通过网络上的机构来合作,我们要做好衡量工作,因为现在的代写机构太多了。那么,英国essay代写哪家靠谱呢?下面我们听听英国代写论文老师是怎么说的。