論文代寫-LRRC8a對血壓的影響研究

英国论文代写
论文通代写作者简介

英国论文代写 论文通

英国论文通 ASSIGNMENT PASS - 论文代写以独特的英国论文代写.英国essay代写和assignment代写专业服务理念,尝试创新的代写形式赢得了英国留学生的口碑.我们代写团队对于代写论文采取多样化的手段.做到了代写论文的原创性和对论文抄袭的杜绝.

19/01/2020

論文代寫-LRRC8a對血壓的影響研究。本研究的第二個目的是通過分析內皮細胞來了解LRRC8a對血壓的潛在影響。本實驗中應測量WT小鼠的血壓,並測量LRRC8a KO小鼠的內皮細胞。為此,將測量基線,血管緊張素ii輸注通過滲透微型泵。滲透微型泵將用於誘發高血壓。通過尾袖血壓測量和植入的主動脈血壓端粒來測量血壓。在此分析之後,我們將對移出的主動脈進行血管反應性研究,分別對p-eNOS、eNOS、pAKT1、pAKT2、AKT1、AKT2、p-p66shc進行免疫染色。然後用內皮一氧化氮和氧化劑對其進行定量。將采用熒光和化學發光方法。接下來論文范文論文代寫-LRRC8a對血壓的影響研究分享給留學生閱讀。

The primary objective of this current proposal is to determine the mechanism by which the LRRC8a regulates endothelium dependent vasorelaxation and blood pressure. In order to test the above hypothesis of LRRC8a, there will be two specific aims that will be analyzed for the research. It is the investigation of the LRRC8a-p66shc-PI3K-AKT-eNOS signaling in endothelium. For this, there should be analysis of the LRRC8a for activation of PI3K-AKT-eNOS and for the suppression of the p66shc pathway. This is done by measuring i pAKT1, pAKT2, AKT1, AKT1, p-eNOS, eNOS, and p-p66shc +/- LRRC8a +/- p66shc under basal resting conditions and in response to endothelial cell stretching on deformable membranes using the FlexCell System. Subsequently, there will be experimentation on HUVEC and in arterial endothelial cells +/- Ad-shSCR/LRRC8a +/- RNAi for p66shc. Added to this the primary cultures of aortic endothelial cells will be isolated from WT and endothelial LRRC8a KO of mice and transgenic mice over expressing shRNA to p66shc. From this, there will be determination made about the LRRC8a forms a molecular complex with p66shc. This is done by co-immunoprecipitation and co-localization studies in endothelial cells.

The second aim of this research is to understand the underlying implication of the LRRC8a on blood pressure by analyzing the endothelial cell. In this sceaniro, there should be measurement of the blood pressure in WT and the LRRC8a KO mice endothelial cells should be measures. For this, there will be measurement of the baseline, Angiotensin-II infusion via osmotic mini-pumps. Osmotic mini-pumps will be used to induce conditions of hypertension. Blood pressure will be measured by using Tail-cuff BP measurement and implantable aortic BP telomeres. If it is observed that LRRC8a KO mice develop HTN then they will be cross analyzed with p66shcRNAi mice. This p66shcRNAi mouse is found to have a global knockdown of p66shc. This is done to determine if this phenotype can be found in LRRC8a KO/p66shcRNAi mice. Subsequent to this analysis the vascular reactivity studies on explanted aortas, immunostaining of p-eNOS, eNOS, pAKT1, pAKT2, AKT1, AKT2, p-p66shc will be done. This would then be and quantified with endothelial NO and oxidants. Fluorescence and chemiluminescence methodologies will be performed. Previous literature of endothelial VRAC has been analyzed over the past 16 years. There is a considerable lack of knowledge about the ion channels. Using the adenoviral shRNA-mediated LRRC8a knock-down it can be established that LRRC8a is required for the volume-regulated anion current (VRAC) in human umbilical vein endothelial cells

論文范文論文代寫-LRRC8a對血壓的影響研究就為留學生分享到這裏。留學生論文不會寫怎么辦?論文代寫推薦英國論文通AssignmentPass網站平台,此論文代寫平台是英國信譽高的留學教育機構,論文寫作專業信譽高,而且論文代寫價格公平合理,為留學生們提供畢業論文代寫、essay代寫、assignment代寫等論文服務,英國論文通AssignmentPass豐富的論文代寫經驗值得留學生選擇和信賴!

相关的论文代写话题 . . .

英国代写论文:如何写论文摘要?

英国代写论文:如何写论文摘要?

英国代写论文:如何写论文摘要?我们在写英国论文的时候,严格意义上讲是一定要写摘要的,没有摘要的论文是不完整的。可以说摘要是论文中很重要的一部分,那么,我们该如何写论文摘要呢?下面听听英国论文代写推荐老师是怎么说的吧。

英国论文代写的注意事项有哪些?

英国论文代写的注意事项有哪些?

每一个留学生都要面对论文的写作,很多人都觉得essay作业繁重,再加上有些比较复杂的essay自己无法完成,只能求助于论文代写机构了。而市场上有很多代写机构,有的服务很不错,有的却敷衍了事。那么,英国论文代写的注意事项有哪些?下面听听英国代写论文老师是怎么说的吧。

英国论文代写有哪些风险?如何规避?

英国论文代写有哪些风险?如何规避?

不管是在国内还是国外,论文代写已经屡见不鲜了。英国的教育资源十分发达,高校对学生的要求也最严格,如果学生找论文代写是要承担一定风险的。那么,英国论文代写有哪些风险?如何规避?下面听听英国代写老师是怎么说的吧。